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Status |
Public on Sep 11, 2016 |
Title |
Adipose_T1_Pre-LPS [Scorr (2599) 30-1] |
Sample type |
RNA |
|
|
Source name |
Human Adipose Pre-LPS
|
Organism |
Homo sapiens |
Characteristics |
tissue: Adipose
|
Treatment protocol |
We administered endotoxin (LPS, 3ng/kg IV) to healthy human volunteers to evoke endotoxemia, and obtained subcutaneous adipose biopsies at 0, 4, 12 and 24 hours post-LPS
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from adipose using the Qiagen Rneasy Lipid tissue Mini kit
|
Label |
biotin
|
Label protocol |
cDNA and cRNA was prepared from 100ng RNA using standard protocols
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|
|
Hybridization protocol |
Samples were hybridized to Affymetrix GeneChip Human Genome U133Plus 2.0 arrays. Microarrays were washed and then stained with amplified fluorescence markers
|
Scan protocol |
Signals were collected on a confocal scanner at 3 μm resolution (excitation at 570 nm)
|
Description |
Gene expression data from human adipose Scorr (2599) 30-1
|
Data processing |
Microarray Suite version 5.0 (MAS 5.0) was used to quantitate expression levels of average signal from two sequential scans. Array signal intensities passed a two-stage QC process including affyQCReport package. All arrays had similar background values and distributions of intensity.
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Submission date |
Jul 05, 2012 |
Last update date |
Sep 11, 2016 |
Contact name |
Chenyi Xue |
Organization name |
Columbia University
|
Street address |
630 W 168th St.
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE39117 |
Expression data from Human Adipose tissue |
GSE39118 |
RNA-Seq and expression data from human adipose tissue |
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