|
| Status |
Public on Sep 11, 2016 |
| Title |
Adipose_T4_Post-LPS [Scorr (2602) 30-4] |
| Sample type |
RNA |
| |
|
| Source name |
Human Adipose 24hours post-LPS
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Adipose
|
| Treatment protocol |
We administered endotoxin (LPS, 3ng/kg IV) to healthy human volunteers to evoke endotoxemia, and obtained subcutaneous adipose biopsies at 0, 4, 12 and 24 hours post-LPS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from adipose using the Qiagen Rneasy Lipid tissue Mini kit
|
| Label |
biotin
|
| Label protocol |
cDNA and cRNA was prepared from 100ng RNA using standard protocols
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix GeneChip Human Genome U133Plus 2.0 arrays. Microarrays were washed and then stained with amplified fluorescence markers
|
| Scan protocol |
Signals were collected on a confocal scanner at 3 μm resolution (excitation at 570 nm)
|
| Description |
Gene expression data from human adipose
Scorr (2602) 30-4
|
| Data processing |
Microarray Suite version 5.0 (MAS 5.0) was used to quantitate expression levels of average signal from two sequential scans. Array signal intensities passed a two-stage QC process including affyQCReport package. All arrays had similar background values and distributions of intensity.
|
| |
|
| Submission date |
Jul 05, 2012 |
| Last update date |
Sep 11, 2016 |
| Contact name |
Chenyi Xue |
| Organization name |
Columbia University
|
| Street address |
630 W 168th St.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE39117 |
Expression data from Human Adipose tissue |
| GSE39118 |
RNA-Seq and expression data from human adipose tissue |
|
