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Sample GSM956402 Query DataSets for GSM956402
Status Public on Sep 11, 2016
Title Adipose_T3_Post-LPS [Scorr (2643) 357-3]
Sample type RNA
 
Source name Human Adipose 12hours post-LPS
Organism Homo sapiens
Characteristics tissue: Adipose
Treatment protocol We administered endotoxin (LPS, 3ng/kg IV) to healthy human volunteers to evoke endotoxemia, and obtained subcutaneous adipose biopsies at 0, 4, 12 and 24 hours post-LPS
Extracted molecule total RNA
Extraction protocol RNA was extracted from adipose using the Qiagen Rneasy Lipid tissue Mini kit
Label biotin
Label protocol cDNA and cRNA was prepared from 100ng RNA using standard protocols
 
Hybridization protocol Samples were hybridized to Affymetrix GeneChip Human Genome U133Plus 2.0 arrays. Microarrays were washed and then stained with amplified fluorescence markers
Scan protocol Signals were collected on a confocal scanner at 3 μm resolution (excitation at 570 nm)
Description Gene expression data from human adipose
Scorr (2643) 357-3
Data processing Microarray Suite version 5.0 (MAS 5.0) was used to quantitate expression levels of average signal from two sequential scans. Array signal intensities passed a two-stage QC process including affyQCReport package. All arrays had similar background values and distributions of intensity.
 
Submission date Jul 05, 2012
Last update date Sep 11, 2016
Contact name Chenyi Xue
Organization name Columbia University
Street address 630 W 168th St.
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platform ID GPL570
Series (2)
GSE39117 Expression data from Human Adipose tissue
GSE39118 RNA-Seq and expression data from human adipose tissue

Data table header descriptions
ID_REF
VALUE log transformed MAS5 signal intensity

Data table
ID_REF VALUE
1007_s_at 9.63161072
1053_at 7.534866897
117_at 8.493443596
121_at 7.036499562
1294_at 8.257155055
1316_at 8.11204186
1320_at 6.537165449
1405_i_at 4.746940351
1487_at 8.810889954
1494_f_at 6.90327188
1552256_a_at 8.261280116
1552257_a_at 9.442457439
1552258_at 6.178813344
1552263_at 5.682040489
1552264_a_at 7.420420819
1552266_at 5.402200595
1552274_at 7.2696146
1552275_s_at 8.855185951
1552277_a_at 7.19680074
1552278_a_at 6.162192001

Total number of rows: 34638

Table truncated, full table size 768 Kbytes.




Supplementary file Size Download File type/resource
GSM956402_Scorr_2643_357-3_U133+v2.CEL.gz 5.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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