|
| Status |
Public on Jul 17, 2015 |
| Title |
AV fistula patient 2 |
| Sample type |
RNA |
| |
|
| Source name |
venous segment of AV fistula
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: venous segment of AV fistula
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We obtained six specimens from overflowing AV fistulae and four from normal control cephalic veins. Surgical specimens were cut into 0.5-cm cubes or smaller and stored in RNA later (Ambion, Austin, TX) overnight at 4 ± 3℃, then stored at –80℃ until RNA extraction.
|
| Label |
Cy3
|
| Label protocol |
Agilent one-color Low RNA Input Linear Amplification Kit labeling protocol
|
| |
|
| Hybridization protocol |
Agilent one-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 5 micron resolution.
|
| Description |
Gene expression of venous segment of AV fistula
|
| Data processing |
Images were quantified using Agilent Feature Extraction software version 10.7.3.1
|
| |
|
| Submission date |
Jul 19, 2012 |
| Last update date |
Jul 17, 2015 |
| Contact name |
Yasuhiro Hashimoto |
| E-mail(s) |
bikkuri@opal.plala.or.jp
|
| Phone |
81-172-87-1221
|
| Fax |
81-172-87-1228
|
| Organization name |
Oyokyo Kidney Research Institute Hirosaki Hospital
|
| Department |
Urology
|
| Street address |
90 Yamazaki Kozawa
|
| City |
Hirosaki |
| State/province |
Aomori |
| ZIP/Postal code |
036-8243 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10332 |
| Series (1) |
| GSE39488 |
Gene expression changes in venous segments of overflowing arteriovenous fistulae |
|
