|
| Status |
Public on Mar 31, 2013 |
| Title |
Meis KO RNAseq 1601 |
| Sample type |
SRA |
| |
|
| Source name |
E11.5 Embryo
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: whole embryo
developmental stage: embryonic day 11.5
genotype/variation: Meis KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was purified from whole E11.5 mouse embryos (Prep1 hypomorphic, Meis1 KO and wt control littermates of each). mRNA was purified and the library for Illumina chromatin sequencing prepared according to the Illumina recommendations. mRNA samples were sequenced using the single-end 68bp protocol. Reads (~7M per sample) were mapped and transcript expression estimated using RSEM.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
All_KOvsWT_filtered_final.xlsx
|
| Data processing |
mapping and transcription estimation with RSEM
Genome_build: mm9
Supplementary_files_format_and_content: Excel spreadsheets containing all Ensembl v63 genes with expression data and fold change between WT and mutants
|
| |
|
| Submission date |
Jul 24, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Daniel Mateos San Martín |
| E-mail(s) |
dmateos@cnic.es
|
| Organization name |
Fundación CNIC
|
| Department |
DBDC
|
| Street address |
Melchor Fernández Almagro 3
|
| City |
Madrid |
| ZIP/Postal code |
28027 |
| Country |
Spain |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE39605 |
TALE transcription factors binding and transcriptional effect [RNA-Seq] |
| GSE39609 |
TALE transcription factors binding and transcriptional effect |
|
| Relations |
| SRA |
SRX170710 |
| BioSample |
SAMN01093711 |
