|
| Status |
Public on Jul 24, 2015 |
| Title |
Leiomyosarcoma_FFPE_2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Leiomyosarcoma tissue, FFPE, 1yr
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
tissue: Leiomyosarcoma tissue
tissue status: FFPE
archival leiomyosarcoma age: 1yr
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extracted using Qiagen DNeasy following manufacturers' instructions for fresh tissue of Agilent protocol for FFPE tissue
|
| Label |
Cy5
|
| Label protocol |
500 - 1000ng DNA was labeled using Agilent ULS Labeling protocol for FFPE tissue DNA and Agilent Enzymatic labeling protocol for Fresh tissue DNA
|
| |
|
| Channel 2 |
| Source name |
Reference DNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
sample type: Reference DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extracted using Qiagen DNeasy following manufacturers' instructions for fresh tissue of Agilent protocol for FFPE tissue
|
| Label |
Cy3
|
| Label protocol |
500 - 1000ng DNA was labeled using Agilent ULS Labeling protocol for FFPE tissue DNA and Agilent Enzymatic labeling protocol for Fresh tissue DNA
|
| |
|
| |
| Hybridization protocol |
Hydridization was done according to Agilent ULS Labeling protocol for FFPE tumour DNA and Agilent Enzymatic Labeling Protocol for fresh tumour DNA
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Description |
LMS 2
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.3) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jul 26, 2012 |
| Last update date |
Jul 24, 2015 |
| Contact name |
Abdulazeez Temitope Salawu |
| E-mail(s) |
mdp09ats@sheffield.ac.uk
|
| Organization name |
University of Sheffield Medical School
|
| Department |
Oncology
|
| Lab |
Rare Tumour Research Group
|
| Street address |
Beech Hill Road
|
| City |
Sheffield |
| ZIP/Postal code |
S10 2RX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10123 |
| Series (1) |
| GSE39663 |
Copy Number Data from Archival Leiomyosarcoma |
|
