|
| Status |
Public on Dec 22, 2016 |
| Title |
MCF7_120days_everolimus |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cell line treated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
cell line orgin: Breast cancer
agent: Everolimus (100 nM)
|
| Treatment protocol |
MCF7 cells were treated with everolimus (100 nM) and HCC1937 cells were treated with everolimus (150 nM) or vehicle (DMSO) for 120 days.
|
| Growth protocol |
MCF7 and HCC1937 cells were cultured and maintained in RPMI medium containing 10% FBS and 2 mM glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
FL-Ovation cDNA Biotin Module V2 from NuGen Technologies Inc
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2)
|
| Scan protocol |
Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2)
|
| Data processing |
The data were analyzed with Partek Genomics Suite using Affymetrix default analysis settings and global scaling as normalization method
|
| |
|
| Submission date |
Jul 27, 2012 |
| Last update date |
Dec 23, 2016 |
| Contact name |
Miguel Angel Pujana |
| E-mail(s) |
mapujana@iconcologia.net
|
| Phone |
0034932607463
|
| Organization name |
Institut Catala d'Oncologia IDIBELL
|
| Lab |
ProCURE
|
| Street address |
Gran Via 199
|
| City |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL15207 |
| Series (2) |
| GSE39693 |
Expression data from MCF7 and HCC1937 cells long-term treated with everolimus |
| GSE39694 |
Expression data from orthotopic tumors and the MCF7 and HCC1937 breast cancer cell lines |
|
