|
Status |
Public on Dec 22, 2016 |
Title |
HCC1937_120days_everolimus |
Sample type |
RNA |
|
|
Source name |
HCC1937 cell line treated
|
Organism |
Homo sapiens |
Characteristics |
cell line: HCC1937 cell line orgin: Breast cancer agent: Everolimus (150 nM)
|
Treatment protocol |
MCF7 cells were treated with everolimus (100 nM) and HCC1937 cells were treated with everolimus (150 nM) or vehicle (DMSO) for 120 days.
|
Growth protocol |
MCF7 and HCC1937 cells were cultured and maintained in RPMI medium containing 10% FBS and 2 mM glutamine.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions
|
Label |
Biotin
|
Label protocol |
FL-Ovation cDNA Biotin Module V2 from NuGen Technologies Inc
|
|
|
Hybridization protocol |
Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2)
|
Scan protocol |
Affymetrix GeneChip Expression Analysis Technical Manual (P/N 702232 Rev 2)
|
Data processing |
The data were analyzed with Partek Genomics Suite using Affymetrix default analysis settings and global scaling as normalization method
|
|
|
Submission date |
Jul 27, 2012 |
Last update date |
Dec 23, 2016 |
Contact name |
Miguel Angel Pujana |
E-mail(s) |
mapujana@iconcologia.net
|
Phone |
0034932607463
|
Organization name |
Institut Catala d'Oncologia IDIBELL
|
Lab |
ProCURE
|
Street address |
Gran Via 199
|
City |
Barcelona |
ZIP/Postal code |
08908 |
Country |
Spain |
|
|
Platform ID |
GPL15207 |
Series (2) |
GSE39693 |
Expression data from MCF7 and HCC1937 cells long-term treated with everolimus |
GSE39694 |
Expression data from orthotopic tumors and the MCF7 and HCC1937 breast cancer cell lines |
|