|
| Status |
Public on Aug 02, 2012 |
| Title |
p53_siTCONS_0004057 |
| Sample type |
RNA |
| |
|
| Source name |
p53_siTCONS0004057
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Hep3B
transfection: siTCONS_00004057
infection: Ad-p53
|
| Treatment protocol |
Hep3B cells were transfected with siRNAs targeting the indicated lincRNAs or negative control. 4h after the transfection, these cells were infected with adenovirus expressing p53 (Ad-p53) or LacZ (Ad-LacZ) as control at 100 moi. 24h after the infection, siRNAs were transfected into cells again.
|
| Growth protocol |
Hep3B cells were cultured in RPMI1640 with 10% FCS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
24h after second transfection, total RNA was extracted by RNeasy mini kit (QIAGEN) according to manufacturer's protocol. RNA was quantified using the NanoDrop spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Description |
Gene expression after the transfection of siTCONS_00004057 and Ad-p53 infection
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction. The VALUEs in the data matrix were calculated by Limma (R package) for the normalization.
|
| |
|
| Submission date |
Jul 31, 2012 |
| Last update date |
Aug 02, 2012 |
| Contact name |
Masashi Idogawa |
| Organization name |
Sapporo Medical University
|
| Street address |
S1W17
|
| City |
Sapporo |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE39773 |
The effect on gene expression by the knockdown of lincRNAs |
|
