|
| Status |
Public on May 13, 2013 |
| Title |
pDR2-anti-Alu_Hygromycin_added->removed-3 |
| Sample type |
RNA |
| |
|
| Source name |
pDR2-anti-Alu_Hygromycin_added->removed
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293
cell type: human embryonic kidney cells
vector transfection: pDR2-anti-Alu
hygromycin treatment: added, then removed
|
| Treatment protocol |
Transfection with null pDR2 vector or pDR2-Alu vector, cultured with Hygromycin selection, then some samples were further cultured without Hygromycin.
|
| Growth protocol |
Maintained in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum and 250 ug of G418 per ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol. An in vitro transcription (IVT) with biotinylated ribonucleotide analog was performed to generate biotin-labeled amplified RNA (aRNA) using the GeneChip 3' IVT Express kit (Affymetrix, Santa Clara, CA). The aRNAs were then purified by magnetic beads and fragmented for the subsequent hybridization according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol. Affymetrix Human PrimeView arrays were used (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
Standard Affymetrix protocol. Fluorescent signal was scanned by GeneChip Scanner 3000 7G (Affymetrix, Santa Clara, CA) to produce digital images and then converted and summarized to intensity readings per probe sets.
|
| Description |
12S219U027
Gene expression data upon transfections.
|
| Data processing |
The entire set of 18 microarrays were normalized by the RMA algorithm using the RMAExpress software.
|
| |
|
| Submission date |
Aug 01, 2012 |
| Last update date |
May 13, 2013 |
| Contact name |
Kung-Hao Liang |
| Organization name |
Chang Gung Memorial Hospital
|
| Street address |
Tung Hwa North Road
|
| City |
Taipei |
| ZIP/Postal code |
10507 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE39822 |
Post-transcriptional regulation by Alu within mRNA |
|
