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Sample GSM980253

Query DataSets for GSM980253

Status

Public on Dec 03, 2012

Title

glioma A60

Sample type

SRA

Source name

Brain

Organism

Homo sapiens

Characteristics

source tissue: Brain
tissue: glioma

Treatment protocol

Study cases were retrieved from the surgical pathology file of the Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taiwan. Patients in this study had provided informed consent, and all procedures were approved by the Institutional Review Board of Taipei Veterans General Hospital. Fresh tumor tissues removed during surgery were snap frozen and kept in liquid nitrogen for RNA extraction.

Extracted molecule

total RNA

Extraction protocol

TruSeq Small RNA Sample Prep Kit (Cat# RS-200-0012) was used with 1 ug of total RNA for the construction of sequencing libraries.

Library strategy

RNA-Seq

Library source

transcriptomic

Library selection

size fractionation

Instrument model

Illumina HiSeq 2000

Data processing

3’ adaptors of the FASTQ raw data were trimmed, and trimmed data were collapsed by the FASTX toolkit (http://hannonlab.cshl.edu/fastx_toolkit).
For all datasets, reads that shorter than 17 nt or longer than 30 nt were filtered out.
Genome_build: hg19
Supplementary_files_format_and_content: RPKM value of mature miRNA across 34 samples

Submission date

Aug 02, 2012

Last update date

May 15, 2019

Contact name

wei-chung cheng

E-mail(s)

d928503@oz.nthu.edu.tw

Organization name

NTHU

Street address

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