|
| Status |
Public on Jan 01, 2014 |
| Title |
C2 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 control cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2C12
type: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol and samples were treated with RiboMinus™ Transcriptome Isolation Kit (Invitrogen). cDNA synthesis and amplification was performed using the GeneChip® Whole Transcript Sense Target Labeling and Control Reagents kit (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Target Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Hybridization was performed as specified, with the GeneChip Hybridization Wash and Stain Kit (Affymetrix)
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Using Affymetrix package (Affy Power Tools) apt-probeset-summarize. Iter-plier algorithm used to quantify probesets.
GPL13185_mjay.pgf
GPL13185_mjay.clf
|
| |
|
| Submission date |
Aug 02, 2012 |
| Last update date |
Jan 01, 2014 |
| Contact name |
Manny Ares |
| Organization name |
UCSC
|
| Department |
Molecular and Cellular Biology
|
| Lab |
Ares
|
| Street address |
1125 High St
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95062 |
| Country |
USA |
| |
|
| Platform ID |
GPL13185 |
| Series (1) |
|
