|
Status |
Public on Aug 07, 2012 |
Title |
Affymetrix HG-U133 arrays of NIH Epigenomics Roadmap Cell Types; H7 (WA07) embryonic stem cells; CE_AM_1013092 |
Sample type |
RNA |
|
|
Source name |
H7 (WA07) embryonic stem cells; NIH Approval # NIHhESC-10-0061; CE_AM_1013092
|
Organism |
Homo sapiens |
Characteristics |
lineage: embrionic stem cell medium: KSR disease: none passage: 46 line: WA-7 differentiation_method: NA batch: WA07-1 biomaterial_type: Cell Line differentiation_stage: undifferentiated Sex: Unknown biomaterial_provider: Harvard University
|
Treatment protocol |
untreated cultured cells
|
Growth protocol |
tissue culture
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were lysed and RNA extracted using Qiagen Rneasy kit
|
Label |
biotin
|
Label protocol |
As recommended by Affymetrix
|
|
|
Hybridization protocol |
As recommended by Affymetrix
|
Scan protocol |
As recommended by Affymetrix
|
Description |
sample_term_id: NTR_0000837 assay_term_id: OBI_0001463 nucleic_acid_term_id: SO_0000356 WA7 stem cells were cultured using standard methods; total RNA was isolated and subject to Affymetrix profiling EDACC Genboree Experiment Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.7559 sample alias: 57 EDACC Genboree Sample Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.5952
|
Data processing |
Processed data were not provided for this record
|
|
|
Submission date |
Aug 06, 2012 |
Last update date |
Jan 29, 2015 |
Contact name |
BROAD INSTITUTE |
E-mail(s) |
rharris1@bcm.tmc.edu
|
Organization name |
Broad Institute
|
Street address |
-
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
Relations |
BioSample |
SAMN00205688 |