|
Status |
Public on Aug 07, 2012 |
Title |
Affymetrix HG-U133 arrays of NIH Epigenomics Roadmap Cell Types; flow sorted CD19+ cells from cord blood; Chuck_Mitch1 |
Sample type |
RNA |
|
|
Source name |
flow sorted CD19+ cells from cord blood; Chuck_Mitch1
|
Organism |
Homo sapiens |
Characteristics |
disease: none biomaterial_provider: FHCRC biomaterial_type: Primary Cell cell_type: CD19 Primary Cells markers: CD19+ donor_id: CD19_0/0/00 donor_age: NA donor_health_status: none donor_sex: Female donor_ethnicity: NA passage_if_expanded: not passaged
|
Treatment protocol |
untreated primary cells
|
Growth protocol |
Uncultured primary cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were lysed and RNA extracted using Qiagen Rneasy kit
|
Label |
biotin
|
Label protocol |
As recommended by Affymetrix
|
|
|
Hybridization protocol |
As recommended by Affymetrix
|
Scan protocol |
As recommended by Affymetrix
|
Description |
sample_term_id: CL_0000236 assay_term_id: OBI_0001463 nucleic_acid_term_id: SO_0000356 CD19+ cells were isolated from umbilical cord blood by flow cytometry; total RNA was isolated and subject to Affymetrix profiling EDACC Genboree Experiment Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.7563 sample alias: 30.0 EDACC Genboree Sample Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.2303
|
Data processing |
Processed data were not provided for this record
|
|
|
Submission date |
Aug 06, 2012 |
Last update date |
Jan 29, 2015 |
Contact name |
BROAD INSTITUTE |
E-mail(s) |
rharris1@bcm.tmc.edu
|
Organization name |
Broad Institute
|
Street address |
-
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
Relations |
BioSample |
SAMN00012125 |