|
| Status |
Public on Oct 10, 2012 |
| Title |
siTINCR repA |
| Sample type |
RNA |
| |
|
| Source name |
Organotypic human epidermis
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: TINCR Knock down A
tissue: Organotypic human epidermis
|
| Treatment protocol |
Nucleofection with siRNA targeting the gene of interest or a non-targeting control
|
| Growth protocol |
Keratinocytes were grown in Gibco SFM medium and nucleofected with a Lonza normal human keratinocyte kit according to manufacturer's protocol. After nucleofection, cells recovered in cell culture for 12-24 hours and then were seeded onto devitalized human dermis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kits were used to isolate total RNA.
|
| Label |
biotin
|
| Label protocol |
Affymetrix 3' IVT Express Kit
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization, Wash, and Stain Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
replicate 2
|
| Data processing |
RMA normalization using justRMA package in R 2.11.1
|
| |
|
| Submission date |
Aug 14, 2012 |
| Last update date |
Oct 11, 2022 |
| Contact name |
Douglas Porter |
| Organization name |
Stanford
|
| Department |
Dermatology
|
| Lab |
Khavari
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (3) |
| GSE40122 |
Control of Somatic Tissue Differentiation by the Long Non-Coding RNA TINCR (array) |
| GSE40123 |
Control of Somatic Tissue Differentiation by the Long Non-Coding RNA TINCR |
| GSE58161 |
Suppression of progenitor differentiation requires the long noncoding RNA ANCR |
|
