|
| Status |
Public on Aug 15, 2013 |
| Title |
Cryptococcus neoformans grown in DMEM for 6hr |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
23904 |
| Tag Length |
17 |
| |
|
| Source name |
Cryptococcus neoformans H99
|
| Organism |
Cryptococcus neoformans var. grubii H99 |
| Characteristics |
mating type: alpha
isolate: human isolate
pathogen: human pathogenic basidomycete yeast
|
| Biomaterial provider |
John Perfect, Duke University, North Carolina, USA
|
| Treatment protocol |
untreated
|
| Growth protocol |
Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FBS), 1% nonessential amino acids, 100 µg mL-1 penicillin-streptomycin, and 4 mM L-glutamine (Invitrogen) for 6hr at 37oC.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
polyA mRNA was extracted using standard TRIZOL procedures and libraries were prepared using the NlaIII anchoring enzyme and the I-Long SAGE kit (Invitrogen).
|
| |
|
| Description |
Cryptococcus neoformans H99
|
| Data processing |
Tag detection and counting was carried out using PHRED and CrossMatch software (University of Oklahoma’s Advanced Center for Genome Technology, http://www.genome.ou.edu/cneo.html)
|
| |
|
| Submission date |
Aug 15, 2012 |
| Last update date |
Aug 15, 2013 |
| Contact name |
Emma Griffiths |
| E-mail(s) |
griffie@msl.ubc.ca
|
| Organization name |
University of British Columbia
|
| Department |
Michael Smith Laboratories
|
| Lab |
Kronstad
|
| Street address |
2185 East Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL15941 |
| Series (1) |
| GSE41066 |
A defect in ATP-citrate lyase links acetyl-CoA production, virulence factor elaboration and virulence in Cryptococcus neoformans |
|
