|
| Status |
Public on Dec 20, 2012 |
| Title |
A549 minus SER 1.5hr compound16 1uM.52 |
| Sample type |
RNA |
| |
|
| Source name |
A549 minusSER 1.5hr compound16 1uM
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
time: 1.5hr
media: NEAA minus Serine
treatment: compound16 1uM
|
| Growth protocol |
A549 cells were grown in RPMI media and switched to BME+NEAA media with or without serine 24 hours prior to compound treatment (1 uM 16 or DMSO). Samples were harvested in triplicate at 0, 1.5, 6, and 24 hours post compound addition, and hybridized to Affymetrix HG-U219 GeneChips.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
standard Affymetix protocol
|
| |
|
| Hybridization protocol |
standard Affymetix protocol
|
| Scan protocol |
standard Affymetix protocol
|
| Description |
A549_minusSER_1.5hr_compound16_1uM
|
| Data processing |
Data were processed using RMA (Gentleman et al., 2004; Irizarry et al., 2003), and differential expression was assessed using the limma package in Bioconductor (Gentleman et al., 2004; Smyth, 2004). Pathway and transcription factor analyses were conducted using the sigpathway algorithm (Tian et al., 2005), Pathway Studio (Ariadne Genomics) and the MetaCore web application (GeneGo Inc).
|
| |
|
| Submission date |
Aug 17, 2012 |
| Last update date |
Dec 20, 2012 |
| Contact name |
Charles Kung |
| E-mail(s) |
charles.kung@agios.com
|
| Organization name |
Agios Pharmaceuticals
|
| Street address |
38 Sidney St., Suite 2
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE40191 |
Small molecule activation of PKM in cancer cells induces serine auxotrophy |
|
