GEO DataSets

(Submitter supplied) We present primary results from the Sequencing Quality Control (SEQC) project, coordinated by the United States Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites using reference RNA samples with built-in controls, we assess RNA sequencing (RNA-seq) performance for sequence discovery and differential expression profiling and compare it to microarray and quantitative PCR (qPCR) data using complementary metrics. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Expression profiling by RT-PCR

4 related Platforms

60 Samples

Download data: CEL, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Rattus norvegicus; synthetic construct; Homo sapiens

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

15 related Platforms

4521 Samples

Download data: CEL, TXT

(Submitter supplied) PCR assay; total volume: 5 μl; instrument: CFX384 (with automation); mastermix: SsoAdvanced SYBR; primer concentration: 250 nM each Samples: cDNA: 25 ng (total RNA equivalents); gDNA: 2.5 ng (Roche); NTC: water + carrier (5 ng/μl yeast transfer RNA); synthetic template (pooled 60-mers in concentration range: 2E7 – 2E1 copies) PCR program: default cycling protocol for SsoAdvanced SYBR (Ta=60°C) Protocol: see manufacturer's website

Organism:

Homo sapiens

2 Series

4 Samples

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