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Links from GEO DataSets

Items: 3

1.

Evaluation of the effectiveness of semen collection and sperm purification methods for spermatozoa transcript profiling

(Submitter supplied) RNA-Seq technique was applied to investigate the effects of two semen collection methods (Pelleted vs Liquefied) and two sperm purification methods (SCLB vs PS) to the integrity of isolated RNAs at different perspectives.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL11154
16 Samples
Download data: BED, TXT
2.

Evaluation of RNA amplification and RNA-Seq library preparation protocols for spermatozoa RNA profiling

(Submitter supplied) RNA-Seq technique was applied to investigate the effects of four cDNA amplification kits and two RNA-Seq library preparation kits to the deep sequencing results at different perspectives.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16791
16 Samples
Download data: BED, XLS
3.

The Transcript Integrity Index (TII) provides a standard measure of sperm RNA

(Submitter supplied) Human sperm RNA-seq was performed and sequences used for the generation of a RNA integrity index capable of assessing samples with high RNA fragmentation. A two-prong approach was used including both manual and computational analysis. This identified a series of sperm RNAs that are consistently intact, and acceptable for use in determining sample RNA quality. These RNAs function in roles related to spermatogenesis and/or fertilization.
Organism:
Homo sapiens
Type:
Other
Platforms:
GPL20301 GPL18573
40 Samples
Download data: TXT
Series
Accession:
GSE201081
ID:
200201081
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