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| Status |
Public on Mar 26, 2018 |
| Title |
Profiling of cytosine DNA-methylation in the Arabidopsis CAF-1 mutant fas2 |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Chromatin Assembly Factor CAF-1 is a histone chaperone involved in nucleosome formation after replication. Here, cytosine DNA-methylation was profiled by bisulphite DNA sequencing in Arabidopsis wild type and the fas2 CAF-1 mutant. To test for transgenerational changes in DNA methylation, fas2 mutants of generation 1 and 6 were compared.
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| Overall design |
Characterisation of the transgenerational methylation changes in fas2-4 mutants using genome-wide bisulfite sequencing. The transgenerational effect was assessed by bisulphite sequencing the first homozygous generation segregated from a selfed heterozygous fas2-4 parental plant and the 6th selfed generation. Col-0 accession was used as a reference. The DNA was sequenced in 100 bp pair-end mode. To increase coverage, mates were mapped as independent single end reads: R1 as directional and R2 in a non-directional.
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| Contributor(s) |
Mozgova I, Wildhaber T, Trejo-Arellano MS, Fajkus J, Roszack P, Köhler C, Hennig L |
| Citation(s) |
29573427 |
| Submission date |
Sep 29, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Minerva Susana Trejo Arellano |
| E-mail(s) |
Minerva.Trejo-Arellano@jic.ac.uk
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| Organization name |
SLU
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| Department |
Plant Biology
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| Lab |
Lars Hennig's Lab
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| Street address |
Almas Allé 5
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| City |
Uppsala |
| State/province |
Uppsala |
| ZIP/Postal code |
75007 |
| Country |
Sweden |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA412659 |
| SRA |
SRP119161 |
