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| Status |
Public on Jan 14, 2019 |
| Title |
Genome-wide transcriptomic analysis of Arabdiopsis mutants controling nuclear size in comparison to the wildtype |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report genome-wide transcriptomic profile of 2 week-old Arabidopsis seedlings from pwo1 and crwn1 crwn2 mutants in comparison to the wildtype (Col-0)
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| Overall design |
Arabidopsis thaliana seedlings of Col-0, pwo1 and crwn1 crwn2 were grown vertically on MS plates in long-day conditions for 2 weeks. Afterwards, RNA from 3 biological replicates per genotype was isolated using RNeasy Plant Mini Kit (Qiagen) and NGS library created using TruSeq RNA Library Prep Kit v2 (Illumina). Paired-end sequencing was performed on HiSeq 2500 platform (Illumina).
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| Contributor(s) |
Mikulski P, Schubert D |
| Citation(s) |
30914470 |
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Pawel Mikulski, Mareike L Hohenstatt, Sara Farrona, Cezary Smaczniak, Kerstin Kaufmann, Gerco Angenent, Daniel Schubert. PWWP INTERACTOR OF POLYCOMBS (PWO1) links PcG-mediated gene repression to the nuclear lamina in Arabidopsis. bioRxiv 220541. doi: 10.1101/220541
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| Submission date |
Nov 07, 2017 |
| Last update date |
Jun 19, 2019 |
| Contact name |
Pawel Mikulski |
| E-mail(s) |
pawel.mikulski@jic.ac.uk
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| Organization name |
John Innes Centre
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| Department |
CDB
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| Street address |
Colney Lane
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| City |
Norwich |
| ZIP/Postal code |
NR4 7UH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA417457 |
| SRA |
SRP124476 |
