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| Status |
Public on Dec 06, 2017 |
| Title |
Introduction of the TERT and BMI1 Genes into Murine Dermal Papilla Cells Ameliorates Hair Inductive Activity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dermal papilla cells (DPCs) are located at the bottom of hair follicles and play important roles on hair induction by interacting with epidermal cells. DPCs are promising cell sources for hair regeneration therapy for alopecia patients. However, freshly isolated DPCs rapidly lose their hair inductive activity and proliferative potential under culture conditions. We examined the effects of telomere reverse transcriptase (TERT) and B-cell-specific Moloney murine leukemia virus insertion region 1 (BMI1) on extending the life span of murine DPCs as well as on hair inductive activity.TERT and BMI1 genes were introduced into murine DPCs using lentivirus vectors. Hair inductive activity of transfected DPCs (t-DPCs) was determined by in vivo chamber assay in nude mice. Transcripts between intact dermal papillae (DPs) and cultured DPCs were compared by microarray analysis.
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| Overall design |
To investigate the hair follicle-related gene expression, transcripts between intact DPs and DPCs at several culture stages [wild-DPC (wP0 and wP5) and t-DPC transfected (tP18)] were compared.
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| Contributor(s) |
Kiso M, Okochi H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 05, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Masahiro Kiso |
| E-mail(s) |
u_manbou2008@yahoo.co.jp
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| Organization name |
National Center for Global Health and Medicine
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| Department |
Regenerative Medicine, Research Institute
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| Street address |
1-21-1 Toyama Shinjuku-ku
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| City |
Tokyo |
| ZIP/Postal code |
162-8655 |
| Country |
Japan |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA421158 |
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