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| Status |
Public on Dec 15, 2017 |
| Title |
Extent and Patterns of MGMT Promoter Methylation in Glioblastoma- and Respective Glioblastoma-Derived Spheres |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by array
|
| Summary |
Quantitative methylation-specific tests suggest that not all cells in a glioblastoma with detectable promoter methylation of the O6-methylguanine DNA methyltransferase (MGMT) gene carry a methylated MGMT allele. This observation may indicate cell subpopulations with distinct MGMT status, raising the question of the clinically relevant cutoff of MGMT methylation therapy. Epigenetic silencing of the MGMT gene by promoter methylation blunts repair of O6-methyl guanine and has been shown to be a predictive factor for benefit from alkylating agent therapy in glioblastoma. Samples of glioblastoma and respective glioblastoma-derived spheres (GS), cultured under stem cell conditions, were analyzed for the degree and pattern of MGMT promoter methylation by methylation-specific clone sequencing, MGMT gene dosage, chromatin status, and respective effects on MGMT expression and MGMT activity.
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| |
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| Overall design |
Copy number information for 4 GBM derived Sphere lines and 2 adherent GBM derived cell line.
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| |
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| Contributor(s) |
Sciuscio D, Bady P, Martinet D, Hegi ME |
| Citation(s) |
21097691 |
| Submission date |
Dec 14, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Pierre Bady |
| E-mail(s) |
pierre.bady@unil.ch
|
| Phone |
0041 21 692 40 95
|
| Organization name |
CHUV / BCF / SIB
|
| Department |
Swiss Institute of Bioinformatics
|
| Lab |
Bioinformatics Core Facility
|
| Street address |
Quartier UNIL-Sorge
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL5477 |
Agilent-014950 Human Genome CGH Microarray 4x44K (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA422445 |
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