|
| Status |
Public on Mar 27, 2018 |
| Title |
Inhibitors of LSD1 target demethylase-independent activity to induce differentiation in acute myeloid leukemia [anti-H3K9 ac, anti-H3K27, RCOR1, SPI1, and MLL4 ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To determine whether changes in histone modifications directly correlate with changes in transcription, THP1 AML cells were treated with a potent and selective LSD1 inhibitor (OG86, 250nM) and then subjected to concomitant RNA sequencing (RNAseq) and ChIP sequencing (ChIPseq) for histone acetylation modifications, RCOR1, SPI1 and MLL4.
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| |
|
| Overall design |
THP1 AML cells were treated with 250nM OG86 or DMSO vehicle for 24 hours in RPMI with 10% fetal bovine serum.
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| |
|
| Contributor(s) |
Somervaille T |
| Citation(s) |
29590629 |
| Submission date |
Mar 20, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Tim C Somervaille |
| E-mail(s) |
tim.somervaille@cruk.manchester.ac.uk
|
| Organization name |
Cancer Research UK Manchester Institute
|
| Lab |
Leukaemia Biology Laboratory
|
| Street address |
Wilmslow Road
|
| City |
Manchester |
| State/province |
Lancashire |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE63222 |
Inhibitors of LSD1 target demethylase-independent activity to induce differentiation in acute myeloid leukemia |
|
| Relations |
| BioProject |
PRJNA439261 |
| SRA |
SRP136095 |
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