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Series GSE114999 Query DataSets for GSE114999
Status Public on Aug 20, 2018
Title Efficient C-to-T base editing in methylated region with human APOBEC3A
Organism Homo sapiens
Experiment type Other
Summary Base editors (BEs) shed new light on correcting disease-related T-to-C mutations. However, current rat APOBEC1-based BEs are less efficient in editing cytosines in highly-methylated regions or in GpC context. By screening a variety of APOBEC/AID deaminases, we showed that human APOBEC3A-conjugated BE and its engineered forms can mediate efficient C-to-T base editing in all examined contexts, including regions with high-methylation levels and GpC dinucleotides, which extends base editing scope.
 
Overall design Examination of indels and base substitutions induced by hA3A-BE3
 
Contributor(s) Yang L, Chen J, Huang X, Yang B, Wang Y, Wang X
Citation(s) 30125268
Submission date May 29, 2018
Last update date Mar 26, 2019
Contact name Li Yang
E-mail(s) liyang_fudan@fudan.edu.cn
Organization name Fudan University
Department Institutes of Biological Sciences
Street address 131 Dong-An Road
City Shanghai
ZIP/Postal code 200032
Country China
 
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (128)
GSM3162370 PDL1-M-b BE3 rep 1
GSM3162371 FANCF-M-b, VEGFA-M-a BE3 rep 1
GSM3162372 FANCF-M-c, MSSK1-M-b, VEGFA-M-b BE3 rep1; PDL1-M-b BE3 rep 2
Relations
BioProject PRJNA473503
SRA SRP149156

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE114999_Indels.xls.gz 23.3 Kb (ftp)(http) XLS
GSE114999_RAW.tar 22.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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