 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 08, 2019 |
| Title |
Epidermal overexpression of ∆NLef1 leads to deregulation of epidermal stem cell fate |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In adult K14ΔNLef1 mouse, the overexpression of ∆NLef1, a B-catenin dominat negative, in basal keratinocytes leads to the conversion of hair follicles into multilayered epithelial cysts and ectopic sebaceous gland. To uncover in vivo changes in gene expression associated to ΔNLef1 activity, we compared the expression profiles of basal keratinocytes (Itga6+) and bulge stem cells (cd34+/itga6+) in wild type and K14ΔNLef1 transgenic mice. Cells were collected from 9.5 week old mice, when the hair follicle are in the resting (telogen) phase of the hair growth cycle. At this stage the ΔNLef1 phenotype is not yet evident enabling us to detect early molecular events.
|
| |
|
| Overall design |
We compared the expression profiles of basal keratinocytes (Itga6+) and bulge stem cells (cd34+/itga6+) in wild type and K14ΔNLef1 transgenic 9.5 week old mice. Three biological replicates for each cell population were analyzed.
|
| |
|
| Contributor(s) |
Donati G, Watt F |
| Citation(s) |
30886049 |
| Submission date |
Aug 03, 2018 |
| Last update date |
May 09, 2019 |
| Contact name |
Giacomo Donati |
| E-mail(s) |
giacomo.donati@kcl.ac.uk
|
| Organization name |
King's College London
|
| Street address |
Guy's Hospital, Great Maze Pond
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL6193 |
[MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version] |
|
| Samples (12)
|
| GSM3318120 |
CD34 ITGA6 double positive keratinocytes from wild type epidermis, biological rep1 |
| GSM3318121 |
CD34 ITGA6 double positive keratinocytes from wild type epidermis, biological rep2 |
| GSM3318122 |
CD34 ITGA6 double positive keratinocytes from wild type epidermis, biological rep3 |
| GSM3318123 |
CD34 ITGA6 double positive keratinocytesfrom K14ΔNLef1 epidermis, biological rep1 |
| GSM3318124 |
CD34 ITGA6 double positive keratinocytesfrom K14ΔNLef1 epidermis, biological rep2 |
| GSM3318125 |
CD34 ITGA6 double positive keratinocytesfrom K14ΔNLef1 epidermis, biological rep3 |
| GSM3318126 |
ITGA6 positive keratinocytes from wild type epidermis, biological rep1 |
| GSM3318127 |
ITGA6 positive keratinocytes from wild type epidermis, biological rep2 |
| GSM3318128 |
ITGA6 positive keratinocytes from wild type epidermis, biological rep3 |
| GSM3318129 |
ITGA6 positive keratinocytesfrom K14ΔNLef1 epidermis, biological rep1 |
| GSM3318130 |
ITGA6 positive keratinocytesfrom K14ΔNLef1 epidermis, biological rep2 |
| GSM3318131 |
ITGA6 positive keratinocytesfrom K14ΔNLef1 epidermis, biological rep3 |
|
| This SubSeries is part of SuperSeries: |
| GSE62608 |
Gata6 network regulates epidermal junctional zone cell fate. |
|
| Relations |
| BioProject |
PRJNA484336 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118073_RAW.tar |
281.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...