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| Status |
Public on Jul 04, 2019 |
| Title |
HPV8 mediated alterations of cellular gene expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We expressed either only the E7 oncoprotein or the complete early genome region (CER) of the human papillomavirus type 8 in primary human adult skin keratinocytes.
Cells were retrovirally transduced, selected, and cellular mRNA was extracted after expansion of the transgene positive keratinocytes.
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| |
|
| Overall design |
Changes in gene expression were measured using the Human Genome cDNA U133A Affymetrix Arrays in biological triplicates in cells containing either the empty vector control (pLXSN), pLXSN-HPV8-E7 or pLXSN-HPV8-CER.
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| |
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| Contributor(s) |
Kirschberg M, Heuser S, Syed AS, Steger G, Hufbauer M, Akgül B |
| Citation(s) |
34552568 |
| Submission date |
Jul 03, 2019 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Matthias Kirschberg |
| E-mail(s) |
matthias.kirschberg@uk-koeln.de
|
| Organization name |
University of Cologne
|
| Department |
Institute of Virology
|
| Lab |
Akgül lab
|
| Street address |
Fürst-Pückler-Str. 56
|
| City |
Cologne |
| State/province |
NRW |
| ZIP/Postal code |
50935 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (9)
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|
| Relations |
| BioProject |
PRJNA552576 |
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