|
Status |
Public on Jul 04, 2019 |
Title |
keratinocyte pLXSN-HPV8-CE #1 |
Sample type |
RNA |
|
|
Source name |
primary human adult skin keratinocytes
|
Organism |
Homo sapiens |
Characteristics |
cell type: keratinocytes isolated from adult skin, retrovirally transduced in passage 1, selected in passage 2, total RNA extracted in passage 3 for analysis
|
Growth protocol |
keratinocytes were cultured on 3T3 feeder cells (passage 0). For retroviral transduction, cells were cultivated in Defined Keratinocyte-SFM (Thermo Fisher Scientific)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total cellular RNA was extracted using the QIAGEN RNeasy Mini Kit
|
Label |
not applicable
|
Label protocol |
standard Affymetrix protocol
|
|
|
Hybridization protocol |
standard Affymetrix protocol
|
Scan protocol |
standard Affymetrix protocol
|
Description |
gene expression data from primary human adult skin keratinocytes expressing the pLXSN-HPV8-CE
|
Data processing |
Data processing was carried using the Transcriptome Analysis Console v.4.0.1.36 and Excel RMA
|
|
|
Submission date |
Jul 03, 2019 |
Last update date |
Jul 04, 2019 |
Contact name |
Matthias Kirschberg |
E-mail(s) |
matthias.kirschberg@uk-koeln.de
|
Organization name |
University of Cologne
|
Department |
Institute of Virology
|
Lab |
Akgül lab
|
Street address |
Fürst-Pückler-Str. 56
|
City |
Cologne |
State/province |
NRW |
ZIP/Postal code |
50935 |
Country |
Germany |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE133813 |
HPV8 mediated alterations of cellular gene expression |
|