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| Status |
Public on May 25, 2022 |
| Title |
Deciphering protective mechanism against human type 2 diabetes through in vitro β cell differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To systemically investigate the role of ZnT8 in β cell maturation, we performed single cell RNA-seq in both WT and KO β cells at both S6 (immature) and S7 (mature) stages. Both WT and KO β cells were obtained from FACS as positive for both INS and NKX6.1. Single cell RNA-seq results revealed that SLC30A8 is mainly involved in β cell maturation process, and further showed that SLC30A8 LOF accelerates β cell maturation and upregulates insulin secretion pathway in mature β cells.
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| Overall design |
In total, 1543 cells with four types of β cell were utilized.
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| Contributor(s) |
Ma Q, Xiao Y |
| Citation(s) |
35842441 |
| Submission date |
Jul 30, 2019 |
| Last update date |
Aug 02, 2022 |
| Contact name |
Menghan Wang |
| E-mail(s) |
wangmhan@gmail.com
|
| Organization name |
Karolinska Institute
|
| Street address |
Karolinska Vägen 6
|
| City |
Stockholm |
| State/province |
please select... |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
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| Platforms (1) |
| GPL15433 |
Illumina HiSeq 1000 (Homo sapiens) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA557351 |
| SRA |
SRP216810 |
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