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| Status |
Public on Dec 18, 2019 |
| Title |
HIF1α Regulates Early Metabolic Changes Due to Activation of Innate Immunity in Nuclear Reprogramming |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Innate immune signaling has recently been shown to play an important role in nuclear reprogramming, by altering the epigenetic landscape and thereby facilitating transcription. However, the mechanisms that link innate immune activation and metabolic regulation in pluripotent stem cells remain poorly defined, particularly with regards to key molecular components. In this study, we show that hypoxia-inducible factor one (HIF1α), a central regulator of adaptation to limiting oxygen tension, is an unexpected but crucial regulator of innate immune-mediated nuclear reprogramming. HIF1α is dramatically upregulated as a consequence of Toll-like receptor 3 (TLR3) signaling and is necessary for efficient induction of pluripotency and transdifferentiation. Bioenergetics studies reveal that HIF1α regulates the reconfiguration of innate immune-mediated reprogramming through its well-established role in throwing a glycolytic switch. We believe that results from these studies can help us better understand the influence of immune signaling in tissue regeneration and lead to new therapeutic strategies.
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| Overall design |
Human fibroblast treated with 30 ng/ml Poly I:C in endothelial cell medium for 0 day, 2 days, 4 days, and 6 days
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| Contributor(s) |
Ruan H, Liu C |
| Citation(s) |
32048999 |
| Submission date |
Dec 17, 2019 |
| Last update date |
Feb 24, 2020 |
| Contact name |
Sayed Nazish |
| E-mail(s) |
sayedns@stanford.edu
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| Organization name |
stanford
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| Street address |
300 Pasteur Dr
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| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA596230 |
| SRA |
SRP237950 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142217_Hif1_D0_D2_D4_D6.csv.gz |
887.0 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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