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| Status |
Public on Aug 10, 2020 |
| Title |
Limited Cell Fluorescence Activated Cell-sort Sequencing (LC-FACSeq) is a method for detecting rare clones in leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Detecting and monitoring tumor clonal evolution in the setting of measurable residual disease is technically difficult due to 1) high sequencing background due to limited numbers of disease cells, 2) loss of tumor genetic material due to the need for high quality purified DNA, and 3) resource and bioinformatics intensive requirements of traditional next generation sequencing techniques. Limited Cell Fluorescence Activated Cell-sort Sequencing (LC-FACSeq), is a fast, reproducible, and cost effective amplicon based sequencing technique that does not require genomic material isolation. It can be used in the context of just a few hundred tumor cells and is easily generalization for monitoring clonal evolution in various different malignancies.
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| Overall design |
Using the LC-FACSeq method to examine clonal evolution in 20 patients treated with ibrutinib for over 4 years (baseline and varying on treatment samples are included for each patient). We validated the reproducibility of LC-FACSeq using different cell titrations (50, 100, 300, 500 cells) of five sorted chronic lymphocytic leukemia (CLL) and 8 sorted acute myeloid leukemia (AML) samples and their corresponding bulk cell samples (100000 cells).
The accompanying manuscript is using these samples to test of novel sequencing technique and the variations identified are from drug treatment studies in patients (already found using alternative methods).
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| Contributor(s) |
Hu EY, Byrd JC, Lozanski G, Muthusamy N |
| Citation(s) |
32554930 |
| Submission date |
Apr 28, 2020 |
| Last update date |
Aug 10, 2020 |
| Contact name |
Eileen Yifan Hu |
| E-mail(s) |
Eileen.hu@osumc.edu
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| Organization name |
The Ohio State University
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| Street address |
Rm 449, 410 West 12th Ave
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| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
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| Platforms (1) |
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| Samples (121)
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| Relations |
| BioProject |
PRJNA629044 |
| SRA |
SRP259016 |
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