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| Status |
Public on Oct 29, 2020 |
| Title |
Targeted RNA-seq signature of Breast Cancer Circulating Tumor Cells correlates with occurrence of bone-only metastases |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Bone is the most frequent site of metastases from breast cancer (BC), although no biomarkers are yet available to predict the risk of skeletal dissemination. Here, we attempted to identify a gene signature correlated with bone metastasis (BM) onset in circulating tumor cells (CTCs) isolated from 40 metastatic BC patients, grouped according to their metastasis sites at the time of enrollment, namely “BM” (bone-only), “ES” (extra-skeletal) or “BM+ES” (bone and extra-skeletal). A 134-gene panel, derived from an extensive literature review, was validated on sub-clones of the MDA-MB-231 BC cell line with variable organotropism and then applied to CTC groups for targeted RNA sequencing. No correlation was found between CTC number and clinico-pathologic variables, whereas 31 differentially expressed genes emerged from “BM” and “ES” CTC comparison. The putative prognostic meaning of the “top-10” most deregulated genes was finally explored and pointed out by employing an external BC dataset.
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| Overall design |
Targeted RNA-seq of circulating tumor cells (CTCs) isolated from 40 metastatic BC patients.
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| Contributor(s) |
Lovero D, D'Oronzo S, Palmirotta R, Cafforio P, Brown J, Wood S, Porta C, Coleman R, Silvestris F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 28, 2020 |
| Last update date |
Oct 29, 2020 |
| Contact name |
Domenica Lovero |
| E-mail(s) |
dom.lovero@gmail.com
|
| Organization name |
University of study of Bari Aldo Modo
|
| Department |
Department of Biomedical Sciences and Human Oncology
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| Lab |
Molecular Medicine
|
| Street address |
P.zza Giulio Cesare, 11
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| City |
Bari |
| State/province |
Bari |
| ZIP/Postal code |
70124 |
| Country |
Italy |
| |
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| Platforms (1) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA672935 |
| SRA |
SRP289479 |
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