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| Status |
Public on Nov 14, 2020 |
| Title |
miRNA expression analysis of DICER1 knock-out cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
To confirm the lack of expression of most miRNAs in DICER1 knock-out cell lines, we performed miRNA microarray analysis.
The purpose of this study is to classify DICER1-dependent Small RNA and independent Small RNA using DICER1 cells using deficient cell lines, and to identify novel small RNA and small RNA processing mechanisms.
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| Overall design |
DICER1 knock-out cell clones (DICER1 KO #1, #2) were made by CRSPR/Cas9 system. The deletion of DICER1 gene were cofirmed by sequence and immunoblotting.
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| Web link |
https://www.cell.com/cell-reports/fulltext/S2211-1247(22)00425-9
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| Contributor(s) |
Ohno S |
| Citation(s) |
35417682 |
| Submission date |
Nov 13, 2020 |
| Last update date |
Apr 14, 2022 |
| Contact name |
Shin-ichiro Ohno |
| Organization name |
Tokyo Medical University
|
| Department |
Molecular Pathology
|
| Street address |
6-1-1 Shinjuku
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| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
1608402 |
| Country |
Japan |
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| Platforms (1) |
| GPL25134 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (miRNA ID version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA678223 |
