|
| Status |
Public on Nov 14, 2020 |
| Title |
NCI-H1299 DICER1 KO #1 |
| Sample type |
RNA |
| |
|
| Source name |
NCI-H1299 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NCI-H1299
cell type: Human non-small cell lung cancer
|
| Growth protocol |
RPMI-1640 supplemented with 10% fetal calf serum, penicillin (100 units/ml), and streptomycin (100 μg/ml)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using the miRNeasy mini kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the miRNA Complete Labeling and hyb Kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
miRNA Complete Labeling and hyb Kit (Agilent)
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2600D SG13054284) using one color scan setting for 1x44k array slides.
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Nov 13, 2020 |
| Last update date |
Nov 14, 2020 |
| Contact name |
Shin-ichiro Ohno |
| Organization name |
Tokyo Medical University
|
| Department |
Molecular Pathology
|
| Street address |
6-1-1 Shinjuku
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
1608402 |
| Country |
Japan |
| |
|
| Platform ID |
GPL25134 |
| Series (1) |
| GSE161400 |
miRNA expression analysis of DICER1 knock-out cell lines |
|
