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| Status |
Public on Apr 21, 2021 |
| Title |
Single-cell atlas of human oral mucosa reveals a stromal-neutrophil axis in tissue immunity regulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The oral mucosa remains an understudied barrier tissue rich in exposure to antigens, commensals and pathogens. Moreover, it is the tissue where one of the most prevalent human microbe-triggered inflammatory diseases, periodontitis, occurs. To understand this complex environment at the cellular level, we assemble herein a human single-cell transcriptome atlas of oral mucosal tissues in health and periodontitis. Our work reveals transcriptional diversity of stromal and immune cell populations, predicts intercellular communication and uncovers an altered immune responsiveness of stromal cells participating in tissue homeostasis and disease at the gingival mucosa. In health, we define unique populations of CXCL1,2,8- expressing epithelial cells and fibroblasts mediating immune homeostasis primarily through the recruitment of neutrophils. In disease, we further observe stromal, particularly fibroblast hyper-responsiveness linked to recruitment of leukocytes and neutrophil populations. Our work suggests a stromal-neutrophil axis as a key regulator of mucosal immunity. Pursuant to these findings, most Mendelian forms of periodontitis were shown to be linked to genetic mutations in neutrophil-expressed genes. Moreover, we document previously unappreciated expression of known pattern- and damage- recognition receptors on stromal cell populations in the setting of periodontitis, suggesting avenues for triggering of stromal responsiveness. This comprehensive atlas offers an important reference for in-depth understanding of oral mucosal homeostasis and inflammation and reveals unique stromal–immune interactions implicated in tissue immunity.
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| Overall design |
Examination of human oral mucosa including healthy and diseased gingival mucosa and healthy buccal mucosa.
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| Contributor(s) |
Williams DW, Moutsopoulos NM |
| Citation(s) |
34129837, 38513665 |
| Submission date |
Jan 04, 2021 |
| Last update date |
May 08, 2024 |
| Contact name |
Niki Moutsopoulos |
| E-mail(s) |
nmoutsopoulos@dir.nidr.nih.gov
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| Organization name |
NIDCR
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| Street address |
30 convent Dr
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| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL30173 |
NextSeq 2000 (Homo sapiens) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA689610 |
| SRA |
SRP300271 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE164241_RAW.tar |
759.5 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
| GSE164241_submission_update.xlsx |
70.6 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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