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| Status |
Public on Mar 10, 2021 |
| Title |
Blood microRNA signatures serve as potential diagnostic biomarkers for hepatic sinusoidal obstruction syndrome caused by Gynura japonica containing pyrrolizidine alkaloids |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Background & Aims: Gynura japonica-induced hepatic sinusoidal obstruction syndrome (HSOS) is closely related with pyrrolizidine alkaloids (PAs) and the prevalence is on the rise worldwide in recent years. However, there is no effective therapy for PA-induced HSOS in clinic, which is partially caused by the failure of quick diagnosis. The aim of the present study was to identify blood miRNAs signatures as the potential biomarkers for PA-induced HSOS in clinic.
Methods: Microarray-based miRNA profiling was performed on blood samples of the discovery cohort, i.e. 9 HSOS patients and 9 healthy donors. The differentially expressed miRNAs were further confirmed using a validation cohort of 20 independent HSOS patients. In addition, rat model was also established by orally administration of total alkaloids extract (TA) from G. japonica to investigate the association of miRNAs biomarkers with the progression of HSOS. Bioinformatic analysis, including GO and KEGG enrichment analyses, receiver operating characteristics curve (ROC) analysis, correlation analysis, etc., were conducted to evaluate the accuracy of the potential miRNA biomarkers.
Results: Three miRNAs, namely miR-148a-3p, miR-362-5p, and miR-194-5p, were over-expressed in PA-induced HSOS patients and rats. They were positively related to the severity of liver injury and displayed considerable diagnostic accuracy for HSOS patients with areas under the curve (AUC) over 0.87. Conclusions: In summary, the present study demonstrated that 3 miRNAs, i.e. hsa-miR-148a-3p, hsa-miR-362-5p, and hsa-miR-194-5p, might serve as potential biomarkers for PA-induced HSOS in clinic.
Conclusions: In summary, the present study demonstrated that 3 miRNAs, i.e. hsa-miR-148a-3p, hsa-miR-362-5p, and hsa-miR-194-5p, might serve as potential biomarkers for PA-induced HSOS in clinic.
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| Overall design |
Microarray-based miRNA profiling was performed on blood samples of the discovery cohort, i.e. 9 HSOS patients and 9 healthy donors.
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| Contributor(s) |
Wang X, Zhuge Y, Xiong A, Yang L, Wang Z |
| Citation(s) |
33679405 |
| Submission date |
Jan 12, 2021 |
| Last update date |
Mar 10, 2021 |
| Contact name |
Xunjiang Wang |
| E-mail(s) |
wangxjknow@126.com
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| Organization name |
Shanghai University of Traditional Chinese Medicine
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| Department |
Shanghai R & D Center for Standardization of Traditional Chinese Medicines
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| Street address |
1200 Cai Lun Road,Zhangjiang Hi-TechPark,Pudong New Area
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| City |
Shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
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| Platforms (1) |
| GPL25134 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (miRNA ID version) |
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| Samples (30)
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| GSM5015733 |
CTRL04: Blood_healthy donors_rep4 |
| GSM5015734 |
CTRL05: Blood_healthy donors_rep5 |
| GSM5015735 |
CTRL06: Blood_healthy donors_rep6 |
| GSM5015736 |
CTRL07: Blood_healthy donors_rep7 |
| GSM5015737 |
CTRL08: Blood_healthy donors_rep8 |
| GSM5015738 |
CTRL09: Blood_healthy donors_rep9 |
| GSM5015739 |
HSOS01: Blood_hepatic sinusoidal obstruction syndrome_rep1 |
| GSM5015740 |
HSOS02: Blood_hepatic sinusoidal obstruction syndrome_rep2 |
| GSM5015741 |
HSOS03: Blood_hepatic sinusoidal obstruction syndrome_rep3 |
| GSM5015742 |
HSOS04: Blood_hepatic sinusoidal obstruction syndrome_rep4 |
| GSM5015743 |
HSOS05: Blood_hepatic sinusoidal obstruction syndrome_rep5 |
| GSM5015744 |
HSOS06: Blood_hepatic sinusoidal obstruction syndrome_rep6 |
| GSM5015745 |
HSOS07: Blood_hepatic sinusoidal obstruction syndrome_rep7 |
| GSM5015746 |
HSOS08: Blood_hepatic sinusoidal obstruction syndrome_rep8 |
| GSM5015747 |
HSOS09: Blood_hepatic sinusoidal obstruction syndrome_rep9 |
| GSM5015748 |
OTHERS01: Other liver disease_rep1 |
| GSM5015749 |
OTHERS02: Other liver disease_rep2 |
| GSM5015750 |
OTHERS03: Other liver disease_rep3 |
| GSM5015751 |
OTHERS04: Other liver disease_rep4 |
| GSM5015752 |
OTHERS05: Other liver disease_rep5 |
| GSM5015753 |
OTHERS06: Other liver disease_rep6 |
| GSM5015754 |
OTHERS07: Other liver disease_rep7 |
| GSM5015755 |
OTHERS08: Other liver disease_rep8 |
| GSM5015756 |
OTHERS09: Other liver disease_rep9 |
| GSM5015757 |
OTHERS10: Other liver disease_rep10 |
| GSM5015758 |
OTHERS11: Other liver disease_rep11 |
| GSM5015759 |
OTHERS12: Other liver disease_rep12 |
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| Relations |
| BioProject |
PRJNA691466 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE164635_Normalized_miRNA_Data.txt.gz |
476.2 Kb |
(ftp)(http) |
TXT |
| GSE164635_RAW.tar |
217.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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