|
| Status |
Public on Mar 10, 2021 |
| Title |
HSOS01: Blood_hepatic sinusoidal obstruction syndrome_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Blood
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Gynura japonica-induced hepatic sinusoidal obstruction syndrome (HSOS)
tissue: whole blood
gender: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A total of 2.5 ml fasting venous blood was collected from each PA-HSOS patient or healthy volunteer and stabilized in PAXgene Blood RNA Tubes (PreAnalytiX, Hombrechtikon, Switzerland). The whole blood was left 2 h at room temperature and stored at -80℃ until subsequent analysis. Total RNA >18 nucleotides (including miRNA) was extracted and purified using PAXgene Blood miRNA Kit (PreAnalytiX, Hombrechtikon, Switzerland) in accordance with the manufacture’s instruction. The extracted RNA was checked for an RNA Integrity Number (RIN) to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). RNA purity and concentration were determined using a Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE).
|
| Label |
Cy3
|
| Label protocol |
Sample tag and reagents are included in the hybrid need Agilent’s miRNA Complete Labeling and Hyb Kit (p/n 5190-0456), Agilent’s miRNA Spike-In Kit (p/n 5190-1934), used for the quality control of marking and hybridization reaction in the experimental process.
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| |
|
| Hybridization protocol |
Each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA) according to the manufacturer’s instructions.
|
| Scan protocol |
Place the chip right in slide holder, place the sample point and face down, and then put it in the scanner. Open the Agilent Scan Control software for 8 x 60 k chips, select AgilentG3_miRNA mode (resolution 3 microns). Set to automatically file access, confirm the scanner status to ready, click Scan Slot m - n, confirm the placement of the chip position. The above steps, when confirmed start scanning chip
|
| Description |
Gene expression of Gynura japonica-induced hepatic sinusoidal obstruction syndrome (HSOS)
|
| Data processing |
Scanning image Feature Extraction are analyzed with Agilent Feature Extraction (AFE) software version 10.7.1.1 or higher outliers were excluded. Get background subtract and space to trend signal strength for processing. Uneven function markers in the feature extraction function of outliers are excluded.
|
| |
|
| Submission date |
Jan 12, 2021 |
| Last update date |
Mar 10, 2021 |
| Contact name |
Xunjiang Wang |
| E-mail(s) |
wangxjknow@126.com
|
| Organization name |
Shanghai University of Traditional Chinese Medicine
|
| Department |
Shanghai R & D Center for Standardization of Traditional Chinese Medicines
|
| Street address |
1200 Cai Lun Road,Zhangjiang Hi-TechPark,Pudong New Area
|
| City |
Shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
| |
|
| Platform ID |
GPL25134 |
| Series (1) |
| GSE164635 |
Blood microRNA signatures serve as potential diagnostic biomarkers for hepatic sinusoidal obstruction syndrome caused by Gynura japonica containing pyrrolizidine alkaloids |
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