|
| Status |
Public on Jul 21, 2021 |
| Title |
Human monoclonal antibodies to M. tuberculosis arabinomannan recognize distinct glycan epitopes - implications for TB diagnostics and research |
| Platform organism |
synthetic construct |
| Sample organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Protein profiling by protein array
|
| Summary |
Human antibody responses to AM/LAM are heterogenous and knowledge of reactivity to specific glycan epitopes at the monoclonal level is limited.
Using novel glycan arrays, we characterized very high affinity monoclonal antibodies to AM/LAM, determined these mAbs are non-competing, and recognized distinct glycan epitopes. distinct from other anti-AM/LAM mAbs reported.
|
| |
|
| Overall design |
Monoclonal antibodies at 5 ug/ml were tested for direct binding to 63 synthetic oligosaccharides
|
| |
|
| Contributor(s) |
Ishida E, Joe M, Lowary TL, Achkar JM |
| Citation(s) |
34642445 |
| Submission date |
Jul 20, 2021 |
| Last update date |
Oct 20, 2021 |
| Contact name |
Jacqueline Achkar |
| E-mail(s) |
jacqueline.achkar@einsteinmed.org
|
| Phone |
7184302971
|
| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 Morris Park Ave.
|
| City |
Bronx |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL30414 |
LAM Subarray 63 - Antigens 10/22/2017 |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA748441 |
