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| Status |
Public on Sep 13, 2021 |
| Title |
Mitogen induced-nucleosomal response pathway and transcriptional programming in mammalian fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Stimulation of the RAS-MAPK pathway activates mitogen- and stress-activated kinases (MSK) 1 and 2, which phosphorylate histone H3 at S10 (H3S10ph) or S28 (H3S28ph) (nucleosomal response) located at the regulatory regions of immediate-early genes, setting in motion a series of chromatin remodeling events that result in transcription initiation. To investigate immediate-early genes regulated by the MSK, we have completed transcriptome analyses (RNA Sequencing) of human normal fibroblast cells (CCD-1070Sk) stimulated with EGF or TPA ± H89, a potent MSK/PKA inhibitor.
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| Overall design |
Treatment of quiescent human cells with fetal bovine serum (FBS), epidermal growth factor (EGF) or the phorbol ester (12-O-tetradecanoylphorbol-13-acetate, TPA) ± H89 following RNA Sequencing.
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| Contributor(s) |
Sharma KL, Jia S, Beacon TH, Adewumi I, López C, Hu P, Xu W, Davie JR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 09, 2021 |
| Last update date |
Jan 16, 2023 |
| Contact name |
James Ronald Davie |
| E-mail(s) |
jim.davie@umanitoba.ca
|
| Phone |
2042723174
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| Organization name |
University of Manitoba
|
| Department |
Biochemistry and Medical Genetics
|
| Street address |
745 Bannatyne Avenue, RM 333A
|
| City |
Winnipeg |
| State/province |
Manitoba |
| ZIP/Postal code |
R3E 0J9 |
| Country |
Canada |
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| Platforms (3) |
| GPL16288 |
AB 5500xl Genetic Analyzer (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA762116 |
| SRA |
SRP336682 |
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