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| Status |
Public on Apr 23, 2024 |
| Title |
miRNA seq mouse liver HFD, PCB126, Aroclor1260 exposure |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Exposure to high fat diet (HFD) and persistent organic pollutants including polychlorinated biphenyls (PCBs) is associated with liver injury in human populations and with non-alcoholic fatty liver disease (NAFLD) and steatohepatitis (NASH) in animal models. Exposure of HFD-fed male mice to the non-dioxin-like (NDL) PCB mixture Aroclor1260 or to dioxin-like (DL) PCB126 or to the combination caused steatohepatitis and differentially altered the liver proteome with pathways involving epigenetic regulation of gene expression. Here unbiased RNA sequencing of miRNA (miRNA-seq) and subsequent network analysis to characterize the biological pathways altered by HFD and PCB exposure compared to HFD alone. Distinct miRNA expression patterns reveald a potential role of miRNAs in the pathogenesis of NAFLD. These results demonstrate miRNA and transcriptome pathways in PCB-related hepatic inflammation and fibrosis in a mouse model of NAFLD.
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| Overall design |
Male C57Bl/6J mice were fed a high fat diet (HFD, 42% kCal from fat) for 12 weeks and exposed to a single oral gavage of vehicle control (corn oil), Aroclor1260 (20 mg/kg), PCB126 (20 µg/kg), or the combination of the doses of Aroclor1260 + PCB126. miRNA was isolated from five mouse livers/experimental exposure using Qiagen miRNA kits. Libraries were prepared from 1 µg of mouse liver RNA the QIAseq miRNA Library Kit. Two sequencing runs were performed on the Illumina NextSeq 500 using the NextSeq 500/550 High Output Kit v2.5 (75 cycles).
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| Contributor(s) |
Petri BJ, Piell KM, Wahlang B, Head KZ, Andreeva K, Rouchka EC, Pan J, Rai SN, Cave MC, Klinge CM |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R21 ES031510 |
m6A Epitranscriptomics in Toxicant Associated Steatohepatitis |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
Carolyn M. Klinge |
| P20 GM103436 |
IDeA Networks of Biomedical Research Excellence in Kentucky |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
MARTHA E BICKFORD |
| R01 ES032189 |
Exposome and Precision Medicine in NAFLD |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
Matthew C Cave |
| R35 ES028373 |
Environmental Liver Disease |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
Matthew C Cave |
| P30 ES030283 |
University of Louisville Center for Integrative Environmental Health Sciences |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
J CHRISTOPHER STATES |
| T32 ES011564 |
UOFL ENVIRONMENTAL HEALTH SCIENCES TRAINING PROGRAM |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
David W Hein |
| P42 ES023716 |
Environmental Exposure and Cardiometabolic Disease |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
Sanjay Srivastava |
| P20 GM113226 |
Administrative Supplement to Hepatobiology and Toxicology COBRE |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
CRAIG J. MCCLAIN |
| P50 AA024337 |
The Role of Nutrition in the Development/Progression of Alcohol-Induced Organ Injury |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
CRAIG J. MCCLAIN |
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| Submission date |
Jan 31, 2022 |
| Last update date |
Apr 23, 2024 |
| Contact name |
Eric Christian Rouchka |
| E-mail(s) |
eric.rouchka@louisville.edu
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| Organization name |
University of Louisville
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| Department |
Biochemistry and Molecular Genetics
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| Lab |
KY INBRE Bioinformatics Core
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| Street address |
522 East Gray Street
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| City |
Louisville |
| State/province |
Kentucky |
| ZIP/Postal code |
40292 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (20)
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| GSM5851530 |
high fat diet control replicate 4 |
| GSM5851531 |
high fat diet control replicate 5 |
| GSM5851532 |
high fat diet + Aroclor1260, replicate 1 |
| GSM5851533 |
high fat diet + Aroclor1260, replicate 2 |
| GSM5851534 |
high fat diet + Aroclor1260, replicate 3 |
| GSM5851535 |
high fat diet + Aroclor1260, replicate 4 |
| GSM5851536 |
high fat diet + Aroclor1260, replicate 5 |
| GSM5851537 |
high fat diet + PCB126, replicate 1 |
| GSM5851538 |
high fat diet + PCB126, replicate 2 |
| GSM5851539 |
high fat diet + PCB126, replicate 3 |
| GSM5851540 |
high fat diet + PCB126, replicate 4 |
| GSM5851541 |
high fat diet + PCB126, replicate 5 |
| GSM5851542 |
high fat diet + Aroclor1260 + PCB126, replicate 1 |
| GSM5851543 |
high fat diet + Aroclor1260 + PCB126, replicate 2 |
| GSM5851544 |
high fat diet + Aroclor1260 + PCB126, replicate 3 |
| GSM5851545 |
high fat diet + Aroclor1260 + PCB126, replicate 4 |
| GSM5851546 |
high fat diet + Aroclor1260 + PCB126, replicate 5 |
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| Relations |
| BioProject |
PRJNA802236 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE195829_rawCounts.txt.gz |
34.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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