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| Status |
Public on May 01, 2023 |
| Title |
Effects of e-cigarette smoke on transcription profiles of PASMCs and AECII in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Cigarette smoke is a major risk factor for the development and progression of chronic obstructive pulmonary disease. Tobacco smoking is also the main cause of lung cancer and the main preventable cause of other lung and heart disease. Strategies to eliminate cigarette smoking have led to the emergence of new tobacco-related products as alternatives for cigarette smoking or tools for smoking cessation. Electronic cigarettes (E-cigarettes) have been introduced to the market in recent years as an alternative to smoking conventional cigarettes. E-cigarettes are nicotine delivery devices by heating and converting to aerosol a liquid mixture composed of propylene glycol, vegetable glycerine, flavouring chemicals, and nicotine. Despite the lack of appropriate studies, e-cigarettes are nowadays widely used and advertised as safe cigarette replacements and the number of users is rapidly growing. Unlike cigarette smoke, where the determinant effects are well studied and documented, very limited amount of research has been done on the health effects and public health of e-cigarettes. The study aims to investigate the effects of e-cigarette vapour extract on gene expression in different primary isolated mouse lung cells [pulmonary arterial smooth muscle cells (PASMC) and alveolar epithelial type two cells (AECII)]. The cells will be exposed to e-cigarette vapour extract (ECVE).
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| Overall design |
Two cell types were treated with 3 conditions. Cells were isolated from 8 mice.
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| Contributor(s) |
Tadele E, Weissmann N, Wilhelm J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 04, 2022 |
| Last update date |
May 03, 2023 |
| Contact name |
Jochen Wilhelm |
| Organization name |
Justus-Liebig University Giessen
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| Department |
Institute for Lung Health
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| Lab |
ILH Genomics
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| Street address |
Aulweg 132
|
| City |
Giessen |
| State/province |
Hessen |
| ZIP/Postal code |
35392 |
| Country |
Germany |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA835068 |
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