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| Status |
Public on Mar 10, 2011 |
| Title |
Genome-wide analysis of mechanosensitive genes using in vivo model of mouse carotid artery endothelium exposed to disturbed flow |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Recently, we have shown that disturbed flow caused by partial ligation of mouse carotid artery rapidly induces endothelial dysfunction and atherosclerosis within two weeks. To understand the molecular mechanisms by which disturbed flow induces atherosclerosis, we carried out genome-wide microarray study using endothelial RNAs isolated from the flow-disturbed left and the contralateral right common carotid artery (LCA and RCA) in C57BL/6 mice.
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| Overall design |
Total intimal RNAs were obtained from LCA and RCA at 12hr and at 48hr post-ligation. Intimal RNAs from three LCAs or RCAs were pooled to obtain ~30ng total RNA.
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| Contributor(s) |
Jo H, Ni C |
| Citation(s) |
20551377 |
| Submission date |
Mar 10, 2010 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Hanjoong Jo |
| E-mail(s) |
hanjoong.jo@bme.gatech.edu
|
| Phone |
404-712-9654
|
| Fax |
404-727-3330
|
| Organization name |
Emory University
|
| Department |
Biomedical Engineering
|
| Street address |
2005 WMB
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30068 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA124983 |
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