|
| Status |
Public on Mar 10, 2011 |
| Title |
LCA EC 48h rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse LCA endothelium 48hr after-ligation
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: Male
tissue: endothelium of carotid, left common carotid artery (LCA)
stress: flow-disturbed (partial ligation)
time: 48 hr
|
| Treatment protocol |
Mice were partially ligated in three of four caudal branches of left common carotid artery (LCA). High-resolution in vivo micro-imaging ultrasound was used to examined the induction of low and oscillatory shear stress in LCA with the contralateral RCA as a control.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from intima were separately obtained from LCA and RCA at 48 hr post-ligation by quickly flushing QIAzol lysis reagent and miRNeasy mini kit was used to RNA extration in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 10, 2010 |
| Last update date |
Mar 10, 2011 |
| Contact name |
Hanjoong Jo |
| E-mail(s) |
hanjoong.jo@bme.gatech.edu
|
| Phone |
404-712-9654
|
| Fax |
404-727-3330
|
| Organization name |
Emory University
|
| Department |
Biomedical Engineering
|
| Street address |
2005 WMB
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30068 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE20741 |
Genome-wide analysis of mechanosensitive genes using in vivo model of mouse carotid artery endothelium exposed to disturbed flow |
|
