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| Status |
Public on May 15, 2024 |
| Title |
Heritable human Polycomb silencing is locus-dependent and requires H2AK119ub1 self-propagation |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Heritable gene silencing is essential for the development of multicellular organisms. Polycomb repressive complexes 1 and 2 (PRC1 and 2) catalyze and recognize histone H2A lysine 119 mono-ubiquitination (H2AK119ub1) and histone H3 lysine 27 trimethylation (H3K27me3), respectively, to mediate heritable gene silencing, but the mechanism of inheritance is not fully understood. Using an inducible gene silencing strategy, we show that the epigenetic inheritance of Polycomb silencing in human cells is strongly dependent on local DNA sequences and chromatin environment. In addition, we find that inheritance is not strictly dependent on H3K27me3 recognition and can be partially achieved by H2AK119ub1 catalysis and recognition, independently of H3K27me3. These findings demonstrate that locus specific features and H2AK119ub1 play key roles in inheritance of Polycomb silencing in human cells.
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| Overall design |
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modification H3K27me3 and Flag for Rescue Constructs Integrated into HEK293FT cells with a 5xtetO-H2B-CITRINE reporter inserted at chr11 (near WT1 gene) or chr 3 (near TFRC gene).
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| Contributor(s) |
Shafiq TA, Moazed D |
| Citation(s) |
38718120 |
| Submission date |
Jul 28, 2022 |
| Last update date |
May 16, 2024 |
| Contact name |
Danesh Moazed |
| Organization name |
Harvard Medical School
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| Department |
Cell Biology
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| Lab |
Moazed Lab
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| Street address |
240 longwood avenue
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (48)
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| GSM6412276 |
WT1,DoxycyclineAddition, H3K27me3, Rep2 |
| GSM6412277 |
WT1,DoxycyclineRemoval, H3K27me3, Rep1 |
| GSM6412278 |
WT1,DoxycyclineRemoval, H3K27me3, Rep2 |
| GSM6412279 |
TFRC, NoDoxycycline, H3K27me3, Rep1 |
| GSM6412280 |
TFRC,DoxycyclineAddition, H3K27me3, Rep1 |
| GSM6412281 |
TFRC,DoxycyclineRemoval, H3K27me3, Rep1 |
| GSM6412282 |
WT1,EEDKO,DoxycyclineAddition, H3K27me3, Rep1 |
| GSM6412283 |
WT1,EEDKO,DoxycyclineAddition, H3K27me3, Rep2 |
| GSM6412284 |
WT1,EEDKO,DoxycyclineRemoval, H3K27me3, Rep1 |
| GSM6412285 |
WT1,EEDKO,DoxycyclineRemoval, H3K27me3, Rep2 |
| GSM6412286 |
WT1,HAEED,DoxycyclineAddition, H3K27me3, Rep1 |
| GSM6412287 |
WT1,HAEED,DoxycyclineAddition, H3K27me3, Rep2 |
| GSM6412288 |
WT1,HAEED,DoxycyclineRemoval, H3K27me3, Rep1 |
| GSM6412289 |
WT1,HAEED,DoxycyclineRemoval, H3K27me3, Rep2 |
| GSM6412290 |
WT1,HAEED_3A,DoxycyclineAddition,H3K27me3, Rep1 |
| GSM6412291 |
WT1,HAEED_3A,DoxycyclineAddition,H3K27me3, Rep1, rerun |
| GSM6412292 |
WT1,HAEED_3A,DoxycyclineAddition,H3K27me3, Rep2 |
| GSM6412293 |
WT1,HAEED_3A,DoxycyclineRemoval, H3K27me3, Rep1 |
| GSM6412294 |
WT1,HAEED_3A,DoxycyclineRemoval, H3K27me3, Rep2 |
| GSM6412295 |
WT1,RING1ABKO,DoxycyclineAddition, H3K27me3, Rep1 |
| GSM6412296 |
WT1,RING1ABKO,DoxycyclineAddition, H3K27me3, Rep2 |
| GSM6412297 |
WT1,RING1ABKO,DoxycyclineAddition, H3K27me3, Rep3 |
| GSM6412298 |
WT1,RING1ABKO,DoxycyclineRemoval, H3K27me3, Rep1 |
| GSM6412299 |
WT1,RING1ABKO,DoxycyclineRemoval, H3K27me3, Rep2 |
| GSM6412300 |
WT1,RING1ABKO,DoxycyclineRemoval, H3K27me3, Rep3 |
| GSM6412301 |
WT1,MTF2KO,DoxycyclineAddition, H3K27me3, Rep1 |
| GSM6412302 |
WT1,MTF2KO,DoxycyclineAddition, H3K27me3, Rep2 |
| GSM6412303 |
WT1,MTF2KO,DoxycyclineRemoval, H3K27me3, Rep1 |
| GSM6412304 |
WT1,MTF2KO,DoxycyclineRemoval, H3K27me3, Rep2 |
| GSM6412305 |
WT1,FlagMTF2,DoxycyclineAddition, H3K27me3, Rep1 |
| GSM6412306 |
WT1,FlagMTF2,DoxycyclineAddition, H3K27me3, Rep2 |
| GSM6412307 |
WT1,FlagMTF2,DoxycyclineRemoval, H3K27me3, Rep1 |
| GSM6412308 |
WT1,FlagMTF2,DoxycyclineRemoval, H3K27me3, Rep2 |
| GSM6412309 |
WT1,FlagMTF2_EH,DoxycyclineAddition, H3K27me3, Rep1 |
| GSM6412310 |
WT1,FlagMTF2_EH,DoxycyclineAddition, H3K27me3, Rep2 |
| GSM6412311 |
WT1,FlagMTF2_EH,DoxycyclineRemoval, H3K27me3, Rep1 |
| GSM6412312 |
WT1,FlagMTF2_EH,DoxycyclineRemoval, H3K27me3, Rep2 |
| GSM6412313 |
WT1,FlagMTF2,DoxycyclineAddition, Flag, Rep1 |
| GSM6412314 |
WT1,FlagMTF2,DoxycyclineAddition, Flag, Rep2 |
| GSM6412315 |
WT1,FlagMTF2,DoxycyclineRemoval, Flag, Rep1 |
| GSM6412316 |
WT1,FlagMTF2,DoxycyclineRemoval, Flag, Rep2 |
| GSM6412317 |
WT1,FlagMTF2_EH,DoxycyclineAddition, Flag, Rep1 |
| GSM6412318 |
WT1,FlagMTF2_EH,DoxycyclineAddition, Flag, Rep2 |
| GSM6412319 |
WT1,FlagMTF2_EH,DoxycyclineRemoval, Flag, Rep1 |
| GSM6412320 |
WT1,FlagMTF2_EH,DoxycyclineRemoval, Flag, Rep2 |
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| Relations |
| BioProject |
PRJNA863276 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE209986_Chr11WT1ReporterInsertion.fasta.gz |
21.1 Kb |
(ftp)(http) |
FASTA |
| GSE209986_Chr3TFRCReporterInsertion.fasta.gz |
24.2 Kb |
(ftp)(http) |
FASTA |
| GSE209986_RAW.tar |
32.5 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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