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| Status |
Public on May 01, 2023 |
| Title |
Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ribosomal frameshifting refers to the process that ribosomes slip into +1 or -1 reading frame, thus produce chimeric trans-frame proteins. In viruses and bacteria, programmed ribosomal frameshifting can produce essential trans-frame proteins for viral replication or regulation of other biological processes. In humans, however, functional trans-frame protein derived from ribosomal frameshifting is scarcely documented. Combining multiple assays, we show that short codon repeats could act as cis-acting elements that stimulate ribosomal frameshifting in humans, abbreviated as CRFS hereafter. Using proteomic analyses, we identified many putative CRFS events from 32 normal human tissues supported by trans-frame peptides positioned at codon repeats. Finally, we show a CRFS-derived trans-frame protein (HDAC1-FS) functions by antagonizing the activities of HDAC1, thus affecting cell migration and apoptosis. These data suggest a novel type of translational recoding associated with codon repeats, which may expand the coding capacity of mRNA and diversify the regulation in human.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data
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| Contributor(s) |
Ren G, Li Z |
| Citation(s) |
38281188 |
| Submission date |
Dec 11, 2022 |
| Last update date |
Apr 04, 2024 |
| Contact name |
Lu Zhang |
| Organization name |
YunNan University
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| Street address |
WuJiaYing Street
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| City |
KunMing |
| State/province |
YunNan |
| ZIP/Postal code |
650500 |
| Country |
China |
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| Platforms (1) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE220678 |
Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human. |
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| Relations |
| BioProject |
PRJNA910966 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE220677_RAW.tar |
243.1 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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