|
| Status |
Public on May 01, 2023 |
| Title |
A549,EV [A549-311-Hyg-11] |
| Sample type |
SRA |
| |
|
| Source name |
A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: lung cancer cell line
genotype: FLAG -OE
treatment: none
|
| Treatment protocol |
HEK293T were transfected using calcium phosphate cell transfection kit
|
| Growth protocol |
HEK293T and A549 cell lines were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using TRIzol reagent, mRNA isolation using NEB Poly(A) mRNA Magnetic Isolation Module(E7490S/L)
VAHTS Universal V8 RNA-seq Library Prep Kit for MGI NRM605
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Data processing |
derRNA using Bowtie2(v2.3.5.1)
The sequenced reads (FATAQ format) are mapped to Homo Sapiens(hg38) genomes using STAR(v2.7.9a).
bamCoverage from deepTools (v3.5.1).
Read count extraction and normalization were performed using featureCounts(v2.0.1)
Supplementary files format and content: hg38
Supplementary files format and content: bigWig
|
| |
|
| Submission date |
Dec 11, 2022 |
| Last update date |
May 02, 2023 |
| Contact name |
Lu Zhang |
| Organization name |
YunNan University
|
| Street address |
WuJiaYing Street
|
| City |
KunMing |
| State/province |
YunNan |
| ZIP/Postal code |
650500 |
| Country |
China |
| |
|
| Platform ID |
GPL30209 |
| Series (2) |
| GSE220677 |
Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human [RNA-seq] |
| GSE220678 |
Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human. |
|
| Relations |
| BioSample |
SAMN32154807 |
| SRA |
SRX18639914 |
