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Series GSE224698 Query DataSets for GSE224698
Status Public on May 01, 2024
Title Gene expression profiles of human dermal fibroblasts and human pluripotent stem cells analyzed by bulk RNA-seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human pluripotent stem cells have two major pluripotent states, primed and naive, and the heterogeneity among cell lines in each pluripotent state remains a major unresolved problem. We showed that the overexpression of H1FOO-DD, which has a short expression period by fusing the destabilized domain to the maternal-specific linker histone H1FOO, together with OCT4, SOX2, KLF4 and LMYC in human somatic cells improves the quality of reprogramming to primed and naive pluripotency.
 
Overall design Cultured human dermal fibroblasts, primed and naive human pluripotent stem cell lines were sampled at specific time points, and analyzed by bulk RNA-seq
 
Contributor(s) Kunitomi A, Kato TM, Nomura M, Yamamoto T
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Feb 07, 2023
Last update date May 01, 2024
Contact name Akira Kunitomi
E-mail(s) akira.kunitomi@gladstone.ucsf.edu
Organization name The J. David Gladstone Institutes
Street address 1650 Owens Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (69)
GSM7029720 Primed OSKL iPSC_1 (bulkRNA-seq)
GSM7029721 Primed OSKL iPSC_2 (bulkRNA-seq)
GSM7029722 Primed OSKL iPSC_3 (bulkRNA-seq)
This SubSeries is part of SuperSeries:
GSE224850 H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency
Relations
BioProject PRJNA932227

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE224698_RAW.tar 45.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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