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Status |
Public on May 15, 2024 |
Title |
Gene expression profile at single cell level of human peripheral blood mononuclear cells cocultured with rotavirus treated or PBS treated human cholangiocyte cell line H69. |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Biliary atresia (BA) is an devastating pediatric cholangiopathy and the leading indication for liver transplant in children worldwide. An in vitro cell system for investigating BA is still lacking. We develop an in vitro cell culture system to mimic immune dysfunction of biliary atresia by coculturing human peripheral blood mononuclear cells (PBMC) with rotavirus treated or PBS treated human cholangiocyte cell line H69. Single cell RNA sequencing analysis of PBMC after coculture was applied to validate the efficacy and fidelity of cell system.
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Overall design |
Human peripheral blood mononuclear cells (PBMC) from healthy donor were coculture with rotavirus (RRV) treated or DMEM medium (control) treated human cholangiocyte cell line H69. Living CD45+ cells were sorted for single cell RNA sequencing. >>>Raw data for human samples not available due to ethical concerns<<<
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Contributor(s) |
Zhenhua L |
Citation(s) |
38678809 |
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Submission date |
Feb 13, 2023 |
Last update date |
May 16, 2024 |
Contact name |
Zhenhua Luo |
E-mail(s) |
luozhh35@mail.sysu.edu.cn
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Organization name |
The First Affiliated Hospital, Sun Yat-sen University
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Street address |
Number 58, zhongshan er road, Yuexiu,
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City |
Guangzhou |
State/province |
Guangzhou |
ZIP/Postal code |
510080 |
Country |
China |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (2) |
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Relations |
BioProject |
PRJNA934292 |