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Series GSE225177 Query DataSets for GSE225177
Status Public on May 15, 2024
Title Gene expression profile at single cell level of human peripheral blood mononuclear cells cocultured with rotavirus treated or PBS treated human cholangiocyte cell line H69.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Biliary atresia (BA) is an devastating pediatric cholangiopathy and the leading indication for liver transplant in children worldwide. An in vitro cell system for investigating BA is still lacking. We develop an in vitro cell culture system to mimic immune dysfunction of biliary atresia by coculturing human peripheral blood mononuclear cells (PBMC) with rotavirus treated or PBS treated human cholangiocyte cell line H69. Single cell RNA sequencing analysis of PBMC after coculture was applied to validate the efficacy and fidelity of cell system.
 
Overall design Human peripheral blood mononuclear cells (PBMC) from healthy donor were coculture with rotavirus (RRV) treated or DMEM medium (control) treated human cholangiocyte cell line H69. Living CD45+ cells were sorted for single cell RNA sequencing.
>>>Raw data for human samples not available due to ethical concerns<<<
 
Contributor(s) Zhenhua L
Citation(s) 38678809
Submission date Feb 13, 2023
Last update date May 16, 2024
Contact name Zhenhua Luo
E-mail(s) luozhh35@mail.sysu.edu.cn
Organization name The First Affiliated Hospital, Sun Yat-sen University
Street address Number 58, zhongshan er road, Yuexiu,
City Guangzhou
State/province Guangzhou
ZIP/Postal code 510080
Country China
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (2)
GSM7040756 PBMC Control
GSM7040757 PBMC RRV
Relations
BioProject PRJNA934292

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Supplementary file Size Download File type/resource
GSE225177_RAW.tar 129.8 Mb (http)(custom) TAR (of MTX, TSV)
Raw data not provided for this record
Processed data provided as supplementary file

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