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| Status |
Public on Apr 17, 2024 |
| Title |
SARS-COV-2 putative mi-RNAs may target host immune system and cellular mechanisms to mediate pathogenesis-an in silico and in vivo study I |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
The exact role of epigenetics in the pathogenesis of severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) is unclear. Herein we have hypothesized that viral micro RNAs (miRNAs) may modulate host cellular and immune responses which may lead to varied clinical presentations. Using bioinformatics tools, we have first predicted putative miRNAs in the genotype of the SARS-CoV-2 and their potential gene targets. Thereafter, quantification of mRNA of key genes regulating inflammation, coagulation, neuropsychiatric functions, fibrosis and glucose metabolism was evaluated in the peripheral blood mononuclear cells and bone marrow of patients suffering from different severities of SARS-CoV-2 infection. Key genes regulating innate immunity, lymphopoiesis, T-cell and B-cell development, cytokine storm, coagulation pathways, fibrosis, neuropsychiatric manifestations, glucose metabolism, and olfaction were significantly downregulated in severe infection when compared with mild infection. Downregulated genes mRNA recovered to normal/near normal levels on recovery from infection. SARS-CoV-2 miRNAs appear promising players in the pathogenesis of COVID-19. Strategies silencing these miRNAs may be explored further as potential therapeutic targets.
Patients with severe illness were defined as: respiratory rate ≥30 per min or SpO2 <90% in ambient air, or acute respiratory distress syndrome or septic shock and those with mild illness as: patients with any of the symptoms like fever, cough, sore throat, malaise, headache, muscle pain, nausea, vomiting, diarrhea, loss of taste and smell, but no dyspnea, or abnormal chest imaging. There were 4 severe SARS CoV-2 patients, Median age 57.5 years (Range 50-72 years), all males, median disease duration 7.5 days, duration of hospital stay 38 days with outcome as 3 deaths and one Post covid lung fibrosis. All 4 mild cases had median age 55.5 years (range 42-0 years), 3 males and one female, mean disease duration 2.8 days, mean duration of hospitalization 14.5 days and all recovered. Median age of all 4 recovered cases was 59 years (range 43-77 years), 3 males and one female, mean disease duration of 6 days, mean disease duration of 22 days and all recovered. There were 4 healthy subjects with median age 50 years (range 42-54 years) and there were 3 males and one female.
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| Overall design |
A total of 12 patients suffereing with SARS-CoV2 infection during first wave (May 2020 - January 2021), 3 during second wave April 2021-Septemer 2021 and 4 healthy controls samples of whole blood in triplicates were analyzed.
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| Contributor(s) |
Rai MK, Tripathy S, Anuja AK, Misra DP, Awale R, Gupta L, Mehta P, Pande G, Sharma S, Hashim Z, Nath A, Gurjar M, Azeem A, R_ N, Gopalakrishnan S, Koshy TN, Singh N, Agarwal V, Khan A, Agarwal V |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 02, 2023 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Vikas Agarwal |
| Organization name |
Sanjay Gandhi Postgraduate Institute of Medical Sciences
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| Department |
Clinical Immunology
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| Street address |
SGPGIMS, Raebareli road
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| City |
Lucknow |
| State/province |
Uttar Pradesh |
| ZIP/Postal code |
226014 |
| Country |
India |
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| Platforms (1) |
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| Samples (57)
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| This SubSeries is part of SuperSeries: |
| GSE231530 |
SARS-COV-2 putative mi-RNAs may target host immune system and cellular mechanisms to mediate pathogenesis-an in silico and in vivo study |
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| Relations |
| BioProject |
PRJNA966240 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231528_CT.txt.gz |
18.6 Kb |
(ftp)(http) |
TXT |
| GSE231528_delta_CT.txt.gz |
2.7 Kb |
(ftp)(http) |
TXT |
| GSE231528_delta_delta_CT.txt.gz |
3.2 Kb |
(ftp)(http) |
TXT |
| GSE231528_fold_change.txt.gz |
4.9 Kb |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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