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| Status |
Public on Apr 26, 2024 |
| Title |
Expression data from leaves of tomato (Solanum lycopersicum L. cv. MicroTom) plants subjected to 1000 Hz sound vibration (at 90 dB for 2 h for 3 consecutive days) and/or inoculated with Pst DC300. |
| Organism |
Solanum lycopersicum |
| Experiment type |
Expression profiling by array
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| Summary |
As mechanical stimuli, sound vibrations are known to influence various molecular and physiological processes that can ultimately improve crop performance and yield.
Microarray analyses were carried out to investigate the global gene expression changes in MicroTom plants subjected to 1000 Hz sound vibration prior to infection with Pst DC300 to test the effectiveness of sound as a priming agent. Data revealed that 1000 Hz pretreatment is effective in eliciting a transcriptional reprogramming in tomato plants grown under normal conditions, but particularly after Pst DC3000 infection. The protective effect of 1000 Hz pretreatment against Pst DC3000 appeared to be mediated by the upregulation of both unique differentially expressed genes (DEGs) involved in cell wall reinforcement, phenylpropanoid pathway and defensive proteins as well as common DEGs associated with boosting plant basal immunity.
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| Overall design |
Gene expression was analyzed in leaves of Micro-Tom plants subjected to 1000 Hz sound vibration and/or inoculated with Pst DC300 in comparison to untreated controls. Leaves were harvested after 1 day and 3 days of sound treatment and immediately frozen in liquid N2 for subsequent RNA extraction. Three biological replicates (each one including four plants) were considered for each treatment, resulting in a total of 24 samples. Microarrays analysis was performed using the GeneChip® Tomato Gene 1.1 ST Array Strip (Affymetrix).
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| Contributor(s) |
Calderon AA, Ferrer MA, Almagro L, Martinez-Calderon A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 27, 2023 |
| Last update date |
Apr 27, 2024 |
| Contact name |
Antonio A Calderón |
| E-mail(s) |
antonio.calderon@upct.es
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| Organization name |
Universidad Politécnica de Cartagena
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| Street address |
Paseo Alfonso XIII, 48
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| City |
Cartagena |
| State/province |
Outside North America |
| ZIP/Postal code |
30203 |
| Country |
Spain |
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| Platforms (1) |
| GPL21525 |
[TomGene-1_1-st] Affymetrix Tomato Genome Array |
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| Samples (24)
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| GSM7665232 |
1000 Hz day1, biological rep 1 |
| GSM7665233 |
1000 Hz day1, biological rep 2 |
| GSM7665234 |
1000 Hz day1, biological rep 3 |
| GSM7665235 |
Pst DC3000, day1, biological rep 1 |
| GSM7665236 |
Pst DC3000, day1, biological rep 2 |
| GSM7665237 |
Pst DC3000, day1, biological rep 3 |
| GSM7665238 |
1000 Hz+Pst day1, biological rep 1 |
| GSM7665239 |
1000 Hz+Pst day1, biological rep 2 |
| GSM7665240 |
1000 Hz+Pst day1, biological rep 3 |
| GSM7665241 |
Control day3, biological rep 1 |
| GSM7665242 |
Control day3, biological rep 2 |
| GSM7665243 |
Control day3, biological rep 3 |
| GSM7665244 |
1000 Hz day3, biological rep 1 |
| GSM7665245 |
1000 Hz day3, biological rep 2 |
| GSM7665246 |
1000 Hz day3, biological rep 3 |
| GSM7665247 |
Pst DC3000, day3, biological rep 1 |
| GSM7665248 |
Pst DC3000, day3, biological rep 2 |
| GSM7665249 |
Pst DC3000, day3, biological rep 3 |
| GSM7665250 |
1000 Hz+Pst day3, biological rep 1 |
| GSM7665251 |
1000 Hz+Pst day3, biological rep 2 |
| GSM7665252 |
1000 Hz+Pst day3, biological rep 3 |
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| Relations |
| BioProject |
PRJNA999320 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE239448_RAW.tar |
113.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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